Caeruloplasmin oxidase activity: measurement in serum by use of o-dianisidine dihydrochloride on a microplate reader

Measurement of ceruloplasmin from its oxidase activity in serum by use of o-dianisidine dihydrochloride.

We have developed a new method for determination of serum ceruloplasmin from its oxidase activity, in which o-dianisidine dihydrochioride is used as substrate. o-Dianisidine dihydrochloride is more stable than is the more widely used p-phenylenediamine substrate, and forms a stable product that is measured at 540 nm. Correlation was good between results of this method and those obtained with bo...

Automated determination of serum ceruloplasmin activity with o-dianisidine dihydrochloride as substrate.

An automated method for the enzymatic determination of ceruloplasmin with o-dianisidine dihydrochloride as substrate is described. The method enables the measurement of 30 samples per hour with a coefficient of variation (day-to-day) of 2.8%. Results correlate well (r equals 0.99 with those obtained by the corresponding manual method

Standardization of serum ceruloplasmin concentrations in international enzyme units with o-dianisidine dihydrochloride as substrate.

We describe a method for calculating the absorptivity (in terms of substrate consumed) of the colored solution obtained when o-dianisidine dihydrochloride is oxidized by ceruloplasmin. By oxidizing o-dianisidine dihydrochloride with known amounts of hydrogen peroxide we could determine that the molar reacting ratio of o-dianisidine to hydrogen peroxide is 2:1. So calculated, absorptivity is 9.6...

Rapid Measurement of Chlorophylls with a Microplate Reader

This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. The procedure enables direct translation of any published equation developed on a 1-cm pathlength spectrophotometer to any format microplate with any sample volume. Chlorophylls were extracted with methanol because it is compatible with cheap polystyrene plates; however, the principles apply t...

Use of glucose oxidase, peroxidase, and O-dianisidine in determination of blood and urinary glucose.